CovToTarget

This tool produce a coverage per feature report based on a target (annotation file in BED or GFF3 format) and the output of covtobed 1.0.

:warning: Note that while bamtocov produces an identical coverage output, it also includes the built-in feature to restrict the analysis to the target.

Help screen

covToTarget $version

  Usage: covtotarget [options] <Target> [<covtobed-output>]

Arguments:                                                                                                                                                 

  <Target>           the BED (or GFF) file containing regions in which to count reads
  <covtobed-output>  covtobed output, or STDIN if not provided

Options:

  -g, --gff                    Force GFF for input (otherwise autodetected by .gff extension)
  -t, --type <feat>            GFF feature type to parse [default: CDS]
  -i, --id <ID>                GFF identifier [default: ID]
  -l, --norm-len               Normalize by gene length
  -b, --bed-output             Output format is BED-like (default is feature_name [tab] counts)
  -h, --help                   Show help

Usage

This program extends covtobed, so it can be used in a stream:

covtobed input/mini.bam | covtotarget input/mini.bed > output/counts.tsv

Output format

A table of features and counts per feature:

target1_8X      699
target2_0X      0
target3_1X      50
for_rev_10Xa    690
for_rev_10Xb    0
for_rev_10Xc    0