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Kaiju

Kaiju can be installed, as usual, from Conda. Like Kraken2, we have access to pre-built databases, and for this tutorial we used the nr 2021-02-24 (52 GB).

Join tabular files

When performing multiple operations on the same dataset, as some of you pointed out during the workshop, we often want to collate metadata. R is the ideal choice for doing...

Merge Reports

Combine single sample abundance estimates into one table

Bracken Abundances

2. Re-estimate kraken2 species abundances using bracken

Taxonomic profiling of whole metagenome shotgun (day 3)

An introduction to the “Tidyverse”

Taxonomic profiling of whole metagenome shotgun (day 2)

Today we will use Braken to recalibrate our estimations, and a set of scripts to merge multiple samples in a single table, and see how to filter it. We will...