Taxonomy placement of viral OTUs
vConTACT2 is a tool to infer the taxonomic relationship of viral sequences with a network-based approach, based on whole genome gene-sharing profiles.
To make use of its output there is a tool called graphanalyzer, and its repository comes with a tutorial.
The programme
- EBAME-22 notes: EBAME-7 specific notes
- Gathering the reads: downloading and subsampling reads from public repositories (optional)
- Gathering the tools: we will use Miniconda to manage our dependencies
- Reads by reads profiling: using Phanta to quickly profile the bacterial and viral components of a microbial community
- De novo mining: assembly based approach, using VirSorter as an example miner
- Viral taxonomy: ab initio taxonomy profiling using vConTACT2
- MetaPhage overview: what is MetaPhage, a reads to report pipeline for viral metagenomics